afamin wnt 3a serum Search Results


l wnt 3a murine fibroblasts  (ATCC)
97
ATCC l wnt 3a murine fibroblasts
L Wnt 3a Murine Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/afamin+wnt+3a+serum/pmc06493770-193-10-14?v=ATCC
Average 97 stars, based on 1 article reviews
l wnt 3a murine fibroblasts - by Bioz Stars, 2026-07
97/100 stars
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recombinant mouse wnt 3a  (Bio-Techne corporation)
96
Bio-Techne corporation recombinant mouse wnt 3a
Recombinant Mouse Wnt 3a, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/afamin+wnt+3a+serum/10__7554_slash_elife__66440-440-18-35?v=Bio-Techne+corporation
Average 96 stars, based on 1 article reviews
recombinant mouse wnt 3a - by Bioz Stars, 2026-07
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wnt3a  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology wnt3a
Wnt3a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/afamin+wnt+3a+serum/pmc02863207-245-12-31?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
wnt3a - by Bioz Stars, 2026-07
93/100 stars
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recombinant wnt 3a  (R&D Systems)
95
R&D Systems recombinant wnt 3a
Recombinant Wnt 3a, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/afamin+wnt+3a+serum/pmc06113213-311-12-14?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
recombinant wnt 3a - by Bioz Stars, 2026-07
95/100 stars
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fetal bovine serum wnt3a conditioned media  (ATCC)
99
ATCC fetal bovine serum wnt3a conditioned media
The listed E6 proteins were co-expressed with FLAG_E6AP_WT, the TOPFLASH or FOPFLASH luciferase reporter, and a renilla luciferase internal transfection control plasmid in C33A cells. Transfected cells were treated with <t>Wnt3A</t> conditioned media for 8.5 hours, lysed in 1X passive lysis buffer (Promega), and measured for luciferase and renilla luminescence. Fold activation was determined by normalizing the TOPFLASH luminescence by the FOPFLASH luminescence. Each E6 protein that could degrade NHERF1 (16E6_WT, 16E6_ΔPBM, 11E6_WT, and 18E6_WT) augmented the canonical Wnt pathway. 16E6_F69A/K72A, which cannot degrade NHERF1, failed to increase Wnt pathway activation over vector levels. Statistical significance was determined from three independent experiments by Student’s t-test (***<0.001, n.s. = no significance).
Fetal Bovine Serum Wnt3a Conditioned Media, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/afamin+wnt+3a+serum/pmc06493770-193-1-14?v=ATCC
Average 99 stars, based on 1 article reviews
fetal bovine serum wnt3a conditioned media - by Bioz Stars, 2026-07
99/100 stars
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bsa solution  (Proteintech)
95
Proteintech bsa solution
The listed E6 proteins were co-expressed with FLAG_E6AP_WT, the TOPFLASH or FOPFLASH luciferase reporter, and a renilla luciferase internal transfection control plasmid in C33A cells. Transfected cells were treated with <t>Wnt3A</t> conditioned media for 8.5 hours, lysed in 1X passive lysis buffer (Promega), and measured for luciferase and renilla luminescence. Fold activation was determined by normalizing the TOPFLASH luminescence by the FOPFLASH luminescence. Each E6 protein that could degrade NHERF1 (16E6_WT, 16E6_ΔPBM, 11E6_WT, and 18E6_WT) augmented the canonical Wnt pathway. 16E6_F69A/K72A, which cannot degrade NHERF1, failed to increase Wnt pathway activation over vector levels. Statistical significance was determined from three independent experiments by Student’s t-test (***<0.001, n.s. = no significance).
Bsa Solution, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/afamin+wnt+3a+serum/pm35551975-120-18-53?v=Proteintech
Average 95 stars, based on 1 article reviews
bsa solution - by Bioz Stars, 2026-07
95/100 stars
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fibroblast growth factor 10  (PeproTech)
90
PeproTech fibroblast growth factor 10
The listed E6 proteins were co-expressed with FLAG_E6AP_WT, the TOPFLASH or FOPFLASH luciferase reporter, and a renilla luciferase internal transfection control plasmid in C33A cells. Transfected cells were treated with <t>Wnt3A</t> conditioned media for 8.5 hours, lysed in 1X passive lysis buffer (Promega), and measured for luciferase and renilla luminescence. Fold activation was determined by normalizing the TOPFLASH luminescence by the FOPFLASH luminescence. Each E6 protein that could degrade NHERF1 (16E6_WT, 16E6_ΔPBM, 11E6_WT, and 18E6_WT) augmented the canonical Wnt pathway. 16E6_F69A/K72A, which cannot degrade NHERF1, failed to increase Wnt pathway activation over vector levels. Statistical significance was determined from three independent experiments by Student’s t-test (***<0.001, n.s. = no significance).
Fibroblast Growth Factor 10, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/afamin+wnt+3a+serum/pm36001976-322-2-9?v=PeproTech
Average 90 stars, based on 1 article reviews
fibroblast growth factor 10 - by Bioz Stars, 2026-07
90/100 stars
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recombinant mouse wnt3a  (R&D Systems)
96
R&D Systems recombinant mouse wnt3a
The listed E6 proteins were co-expressed with FLAG_E6AP_WT, the TOPFLASH or FOPFLASH luciferase reporter, and a renilla luciferase internal transfection control plasmid in C33A cells. Transfected cells were treated with <t>Wnt3A</t> conditioned media for 8.5 hours, lysed in 1X passive lysis buffer (Promega), and measured for luciferase and renilla luminescence. Fold activation was determined by normalizing the TOPFLASH luminescence by the FOPFLASH luminescence. Each E6 protein that could degrade NHERF1 (16E6_WT, 16E6_ΔPBM, 11E6_WT, and 18E6_WT) augmented the canonical Wnt pathway. 16E6_F69A/K72A, which cannot degrade NHERF1, failed to increase Wnt pathway activation over vector levels. Statistical significance was determined from three independent experiments by Student’s t-test (***<0.001, n.s. = no significance).
Recombinant Mouse Wnt3a, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/afamin+wnt+3a+serum/us11834722-167-41-44?v=R%26D+Systems
Average 96 stars, based on 1 article reviews
recombinant mouse wnt3a - by Bioz Stars, 2026-07
96/100 stars
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wnt3a conditioned media  (ATCC)
98
ATCC wnt3a conditioned media
The listed E6 proteins were co-expressed with FLAG_E6AP_WT, the TOPFLASH or FOPFLASH luciferase reporter, and a renilla luciferase internal transfection control plasmid in C33A cells. Transfected cells were treated with <t>Wnt3A</t> conditioned media for 8.5 hours, lysed in 1X passive lysis buffer (Promega), and measured for luciferase and renilla luminescence. Fold activation was determined by normalizing the TOPFLASH luminescence by the FOPFLASH luminescence. Each E6 protein that could degrade NHERF1 (16E6_WT, 16E6_ΔPBM, 11E6_WT, and 18E6_WT) augmented the canonical Wnt pathway. 16E6_F69A/K72A, which cannot degrade NHERF1, failed to increase Wnt pathway activation over vector levels. Statistical significance was determined from three independent experiments by Student’s t-test (***<0.001, n.s. = no significance).
Wnt3a Conditioned Media, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/afamin+wnt+3a+serum/pmc13084684-27-18-30?v=ATCC
Average 98 stars, based on 1 article reviews
wnt3a conditioned media - by Bioz Stars, 2026-07
98/100 stars
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a te 2d modified medium  (ATCC)
98
ATCC a te 2d modified medium
The listed E6 proteins were co-expressed with FLAG_E6AP_WT, the TOPFLASH or FOPFLASH luciferase reporter, and a renilla luciferase internal transfection control plasmid in C33A cells. Transfected cells were treated with <t>Wnt3A</t> conditioned media for 8.5 hours, lysed in 1X passive lysis buffer (Promega), and measured for luciferase and renilla luminescence. Fold activation was determined by normalizing the TOPFLASH luminescence by the FOPFLASH luminescence. Each E6 protein that could degrade NHERF1 (16E6_WT, 16E6_ΔPBM, 11E6_WT, and 18E6_WT) augmented the canonical Wnt pathway. 16E6_F69A/K72A, which cannot degrade NHERF1, failed to increase Wnt pathway activation over vector levels. Statistical significance was determined from three independent experiments by Student’s t-test (***<0.001, n.s. = no significance).
A Te 2d Modified Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/afamin+wnt+3a+serum/bio_rxiv__64898__2026__03__26__714557-259-5-24?v=ATCC
Average 98 stars, based on 1 article reviews
a te 2d modified medium - by Bioz Stars, 2026-07
98/100 stars
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human wnt3a  (R&D Systems)
97
R&D Systems human wnt3a
The listed E6 proteins were co-expressed with FLAG_E6AP_WT, the TOPFLASH or FOPFLASH luciferase reporter, and a renilla luciferase internal transfection control plasmid in C33A cells. Transfected cells were treated with <t>Wnt3A</t> conditioned media for 8.5 hours, lysed in 1X passive lysis buffer (Promega), and measured for luciferase and renilla luminescence. Fold activation was determined by normalizing the TOPFLASH luminescence by the FOPFLASH luminescence. Each E6 protein that could degrade NHERF1 (16E6_WT, 16E6_ΔPBM, 11E6_WT, and 18E6_WT) augmented the canonical Wnt pathway. 16E6_F69A/K72A, which cannot degrade NHERF1, failed to increase Wnt pathway activation over vector levels. Statistical significance was determined from three independent experiments by Student’s t-test (***<0.001, n.s. = no significance).
Human Wnt3a, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/afamin+wnt+3a+serum/pm34988666-39-56-58?v=R%26D+Systems
Average 97 stars, based on 1 article reviews
human wnt3a - by Bioz Stars, 2026-07
97/100 stars
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fibroblast growth factor 10 peprotech 10026  (PeproTech)
90
PeproTech fibroblast growth factor 10 peprotech 10026
The listed E6 proteins were co-expressed with FLAG_E6AP_WT, the TOPFLASH or FOPFLASH luciferase reporter, and a renilla luciferase internal transfection control plasmid in C33A cells. Transfected cells were treated with <t>Wnt3A</t> conditioned media for 8.5 hours, lysed in 1X passive lysis buffer (Promega), and measured for luciferase and renilla luminescence. Fold activation was determined by normalizing the TOPFLASH luminescence by the FOPFLASH luminescence. Each E6 protein that could degrade NHERF1 (16E6_WT, 16E6_ΔPBM, 11E6_WT, and 18E6_WT) augmented the canonical Wnt pathway. 16E6_F69A/K72A, which cannot degrade NHERF1, failed to increase Wnt pathway activation over vector levels. Statistical significance was determined from three independent experiments by Student’s t-test (***<0.001, n.s. = no significance).
Fibroblast Growth Factor 10 Peprotech 10026, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/afamin+wnt+3a+serum/pm35187519-495-102-108?v=PeproTech
Average 90 stars, based on 1 article reviews
fibroblast growth factor 10 peprotech 10026 - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


The listed E6 proteins were co-expressed with FLAG_E6AP_WT, the TOPFLASH or FOPFLASH luciferase reporter, and a renilla luciferase internal transfection control plasmid in C33A cells. Transfected cells were treated with Wnt3A conditioned media for 8.5 hours, lysed in 1X passive lysis buffer (Promega), and measured for luciferase and renilla luminescence. Fold activation was determined by normalizing the TOPFLASH luminescence by the FOPFLASH luminescence. Each E6 protein that could degrade NHERF1 (16E6_WT, 16E6_ΔPBM, 11E6_WT, and 18E6_WT) augmented the canonical Wnt pathway. 16E6_F69A/K72A, which cannot degrade NHERF1, failed to increase Wnt pathway activation over vector levels. Statistical significance was determined from three independent experiments by Student’s t-test (***<0.001, n.s. = no significance).

Journal: PLoS Pathogens

Article Title: E6 proteins from high-risk HPV, low-risk HPV, and animal papillomaviruses activate the Wnt/β-catenin pathway through E6AP-dependent degradation of NHERF1

doi: 10.1371/journal.ppat.1007575

Figure Lengend Snippet: The listed E6 proteins were co-expressed with FLAG_E6AP_WT, the TOPFLASH or FOPFLASH luciferase reporter, and a renilla luciferase internal transfection control plasmid in C33A cells. Transfected cells were treated with Wnt3A conditioned media for 8.5 hours, lysed in 1X passive lysis buffer (Promega), and measured for luciferase and renilla luminescence. Fold activation was determined by normalizing the TOPFLASH luminescence by the FOPFLASH luminescence. Each E6 protein that could degrade NHERF1 (16E6_WT, 16E6_ΔPBM, 11E6_WT, and 18E6_WT) augmented the canonical Wnt pathway. 16E6_F69A/K72A, which cannot degrade NHERF1, failed to increase Wnt pathway activation over vector levels. Statistical significance was determined from three independent experiments by Student’s t-test (***<0.001, n.s. = no significance).

Article Snippet: 10% fetal bovine serum Wnt3A conditioned media was generated using L Wnt-3A murine fibroblasts (ATCC, CRL-2647) as previously described [ ].

Techniques: Luciferase, Transfection, Control, Plasmid Preparation, Lysis, Activation Assay